## Thermal Stress and Crab Chemical Cues

An experiment studying the effect of thermal stress on chemical cue reception and response time in the hairy shorecrab _Hemigrapsis oregonensis_.

METHODS:
Crabs were first selected for the two temperature tanks: one control tank held at 14°C, and an elevated temperature tank at 29°C. 
  - Before long-term temperature exposure, crabs were weighed and an ID picture was taken.
  - Approximate carapace width was taken using a ruler (more detailed carapace measurements to come with ImageJ). 
  - A blue numbered tag was attached to each crab
  - RESAZURIN ASSAY: After 10 minutes of acclimation post-weighing, crabs underwent their first Resazurin assay to establish baseline respirometry.
      - All 12 crabs were placed in a well plate containing 8mL Resazurin solution
      - T0 measurements were taken for each crab's well, and for one empty control well
      - Measurements were taken each half hour, for a total of 4 measurements per crab (T0 - T3).
